ABSTRACT The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.

ABSTRACT The objective of this study was to evaluate the potential of rooting of podocarpos cuttings in function of the number of leaves, concentrations of IBA and phytorregulators, application methods and cutting season. Two experiments were carried out under weaning conditions, in a factorial scheme containing 3 replicates of 15 cuttings, the first with 4 concentrations of IBA, 3 leaves at the cuttings and 2 seasons. These were removed in August/2016 and January/2017, prepared with 10 cm in length and immersed in solutions for 10 s. In the second experiment three types of phytoregulators and 2 application forms were tested (slow and quick immersion). Cuttings were prepared in the same manner as in the first experiment, maintaining 4 leaves. The bases of the cuttings were immersed for 10 s and 24 h in the solutions, then taken to root. After 100 days of experimentation, it was concluded that cuttings collected in the summer had a higher percentage of rooting, 4 leaves and IBA concentration should be maintained between 750 and 800 mg L-1. The best form of application of phytoregulator is by rapid immersion.

Resumo Como a região oeste do Paraná é considerada subtropical, com altas temperaturas no verão, os híbridos de Encyclia são comercializados pela beleza de suas flores e durabilidade. No entanto, não existem estudos envolvendo plantas em vasos, tipos de substratos e fertilizantes, além dos híbridos para o gênero Encyclia. Diante do exposto, o objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e o florescimento de Encyclia com base na adubação e no substrato.O experimento foi conduzido em um produtor comercial, de janeiro a setembro de 2017. As mudas permaneceram em ambiente protegido com 50% de sombreamento. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4 x 5 (substratos x intervalos de fertilização), com 4 repetições por tratamento e 4 vasos por repetição. O lote escolhido era oriundo de semeadura in vitro, com o híbrido Encyclia “Kropp” x E. atropurpurea. Após cinco meses da última fertilização, foram realizadas avaliações relacionadas ao desenvolvimento vegetativo e floração. Os substratos à base de esfagno apresentaram melhores resultados no florescimento. As adubações orgânicas com Bokashi aos 45 dias após o transplante (DAT) favorecem a floração e o desenvolvimento vegetativo do híbrido de Encyclia, ocorrendo em vasos contendo substratos à base de esfagno e com adubações orgânicas acima de 60 DAT.

Abstract Due to the fact that the western region of Paraná is considered subtropical, with high temperatures in the summer, the hybrids of Encyclia are commercialized by the beauty of its flowers and durability. However, there are no studies involving potted plants, substrate types and fertilizers, as well as hybrids, for the genus Encyclia. Thus, the objective of this work was to evaluate the vegetative development and flowering of Encyclia based on fertilization and substrate. The experiment was conducted in a commercial orchard, from January to September/2017. The seedlings remained in a protected environment, with 50% shading. The experimental design was randomized blocks, in a 4 x 5 factorial scheme (substrates x fertilization intervals), with 4 replicates per treatment, and 4 pots per replicate. The lot chosen was sowed in vitro, with the hybrid Encyclia “Kropp” x E. atropurpurea. After five months of the last fertilization, the evaluations related to vegetative development and flowering were carried out. Substrates based on sphagnum presented better results in flowering. Organic fertilizations with Bokashi at 45 days after transplant (DAT) favor flowering and vegetative development of the Encyclia hybrid, which occurs in pots containing sphagnum-based substrates and with organic fertilizers above 60 DAT.

RESUMO: Objetivou-se com o presente trabalho avaliar a composição, aspectos nutricionais e análise sensorial da geleia de dovyalis. Frutos de dovyalis foram colhidos de plantas de quatro anos, no pomar da Fazenda Experimental da Unioeste. Estes, foram coletados de manhã, em caixas de polietileno, quando encontravam-se maduros e com coloração arroxeada do epicarpo, sendo imediatamente após, transportados ao laboratório, para o preparo das geleias. Após a chegada dos frutos, estes foram selecionados por tamanho, coloração e peso e, em seguida, higienizados em água corrente e retirados os cálices dos frutos. As avaliações sensoriais das amostras de geleia foram realizadas de forma casualizada, com 30 provadores voluntários. Os provadores foram previamente orientados a avaliarem a aceitação das geleias e suas intenções de compra. Frutos in natura e geleia de dovyalis são fontes de vitamina C. Frutos de dovyalis podem ser usados no processamento, devido sua acidez. A geleia preparada com frutos de dovyalis sem epicarpo e endocarpo, e menor quantidade de açúcar, foi mais apreciada pelos provadores.

ABSTRACT: The objective of this study is to evaluate the composition, nutritional aspects and sensory analysis of the dovyalis jam. Dovyalis fruits were harvested from 4-year-old plants in the orchard of the Unioeste Experimental Farm. They were collected in the morning in polyethylene boxes, when they were ripe and with a purple coloration from the epicarp, being immediately transported to the Laboratory to prepare the jams. After fruits arrival, they were selected by size, color and weight and then sanitized in running water and the cups of the fruits were removed. The sensory evaluations of the jam samples were performed in a randomized fashion, with 30 volunteer tasters. The tasters were previously advised to evaluate the acceptance of the jams and their purchase intentions. Fruits in natura and dovyalis jam are sources of vitamin C. Dovyalis fruits can be used in the processing, due to its acidity. The jam prepared with dovyalis fruits without epicarp and endocarp, and less sugar, was more appreciated by the tasters.

RESUMO: Objetivou-se com o presente trabalho avaliar duas fontes alcoólicas no preparo, caracterização química e aceitabilidade de licores artesanais de doviális. Frutos de doviálisforam colhidos de plantas de 4 anos e imediatamente transportados ao laboratório. Chamou-se de L1 o licor que fora preparado com vodca + frutos sem epicarpo e L2 o licor preparado com aguardente de cana-de-açúcar + frutos com epicarpo e fatiados. Na sequência realizou-se a etapa de infusão ou maceração alcoólica, misturando os frutos + líquido alcoólico. Após o preparo do extrato alcoólico, foi realizada a caracterização química dos produtos finais e, após 60 dias, a análise sensorial e aceitação do produto. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2 x 2 (2 tipos de frutos x 2 tipos de álcool), contendo três amostras de licores por repetição e seis repetições por parcela experimental. Os licores se enquadraram nos parâmetros previstos pela legislação brasileira na avaliação química dos produtos. Quanto a análise sensorial, houve boa aceitabilidade dos provadores do L1 e intenção de compra de 71% dos provadores.

ABSTRACT: This study evaluated two alcoholic sources in the preparation, chemical characterization, and acceptability of homemade Kei apple liqueurs. Kei apple fruits were harvested from 4-year-old plants and immediately transported to the laboratory. The liqueur prepared with vodka + fruits without the epicarp was named L1;the liqueur prepared with sugarcanespirit+ fruits with the epicarp and sliced was named L2. The infusion or alcoholic maceration stage was then performed, mixing the fruits with the alcoholic liquid. After the preparation of the alcoholic extract, the chemical characterization of the final products was performedand after 60 days, the sensory analysis and acceptabilityof the product were conducted. The experimental design was entirely randomized, in a 2 × 2factorial scheme (two types of fruits ×.two types of alcohol), containing three samples of liqueurs per replicate and six replicates per experimental plot. The liqueurs suited the parameters fixed by the Brazilian legislation for the chemical evaluation of these products. As for sensory analysis, L1 had good acceptability from tasters, with apurchase intention by 71% of them.

RESUMO: A propagação assexuada de coníferas, como o Podocarpus macrophyllus, apresenta dificuldades. Entre os fatores que podem tornar mais eficientes a propagação assexuada tem-se o tipo da estaca utilizada, lesões na base da estaca e uso de fitoreguladores, como o ácido indolbutírico (AIB). Objetivou-se determinar o tipo de lesão a ser efetuado na base da estaca e o tipo de estaca bem como método de veiculação do AIB na base da estaca e a concentração do AIB visando obter melhor enraizamento para a espécie. Dois experimentos foram realizados, no período de março a julho de 2018, em blocos casualizados e esquema fatorial, sendo o primeiro 2 x 4 [aplicação AIB em solução e em talco x concentração de 0, 500, 1000 e 1500 mg L-1 de ácido indolbutírico (AIB)] e o segundo 2 x 3 [estaca basal e apical x lesões na base da estaca do tipo bisel, cunha e horizontal].Em ambos experimentos as estacas utilizadas foram dispostas em canteiro de alvenaria contendo areia de textura média. Após 120 dias de experimentação conclui-se que o uso de estacas basais com lesões na base da estaca em cunha e tratadas com AIB em solução na concentração de aproximadamente 550 mL L-1favorecem a porcentagem de enraizamento.

ABSTRACT: The asexual propagation of conifers, such as Podocarpus macrophyllus (Thunb.) Sweet, faces difficulties. Among the factors that can improve the efficiency of asexual propagation are the types of cuttings used and lesions performed at the base of the cuttings, and the use of plant regulators, such as indolebutyric acid (IBA). This study determined the types of lesions to be performed at the cutting base, the type of cuttings used, the method to convey IBA at the cutting base, and the concentration of IBA required to obtain a proper rooting condition for the species. Two experiments were performed from March to July, 2018, in randomized blocks and with a factorial scheme, as follows: 2 x 4 (IBA application in solution and talc x IBA at concentrations of 0, 500, 1,000, and 1,500 mg L-1 IBA); and 2 x 3 (basal and apical cuttings x bevel, wedge, and horizontal lesions at the cutting base). In both experiments, the cuttings used were arranged on a masonry bed containing medium-textured sand. After 120 days of experimentation, the study concluded that the rooting percentage was favored by the use of basal cuttings with wedge lesions at the cutting base and treated with IBA in solution at a concentration of approximately 550 mg L-1.

ABSTRACT The purpose of this study was to evaluate the agronomic behavior of the Niagara Rosada variety cultivated under plastic cover, using defoliation intensities, and its influence on the Plasmopara viticola. The experiment was conducted in Francisco Beltrão, PR, Brazil, in a vineyard of Niagara Rosada variety in a trellised system during the 2016/2017 and 2017/2018 crop seasons. The experimental design consisted of randomized blocks, in a 5 x 2 factorial scheme being five percentages of defoliation (0%, 10%, 20%, 30% and 40%,) and use of coverage (with and without coverage), with three blocks of three useful plants per experimental plot. The temperature, air humidity and incidence of mildew on the bunches and leaves were recorded. At the end of each crop station, three bunches per plant of each plot were evaluated for the fresh biomass of the bunches, length and width of the bunches, soluble solids content, pH, number of bunches per plant, production, and productivity. It was concluded that the plastic cover associated with the environmental conditions, and phytosanitary management can favor the physical-chemical quality and reduce the severity of the mildew in the Niagara Rosada variety providing greater production and productivity. The use of defoliation does not favor the cultivation of this variety in southwestern Paraná.